· polyacrylamideKo te reera me te monomer acrylamide, nga mea timatanga polymerization, te whakakorikori, me te tika o te tote me te ranunga parepare.
· acrylamideme te BIS (N, N '- methylene acrylamide rua) ko te ahua o te monomer matrix gel.
· te haukinia persulfate ka timata te tukanga polymerization whakapiri.Ko te hanga o te kapia me 10% te wairewa haukinia persulfate kua rite ki te wai.Ko te nuinga o nga korero e tohu ana me kaha te whakamahi.Heoi, ko te otinga 10% ka taea te whakanoho ki te 4 ℃ mo etahi wiki kaore he mate nui.Hangaia ki te 10 ml ka peia ina kore te kapia e whakahiato.
Aki: Te ōrau oacrylamidei roto i te whakaraupapa kāpia me te kāpia pūmua e kore e rite.Mena kei te whakamahi i te acrylamide kua oti te hanga: te otinga BIS, kia mohio koe ki te tiki i te pounamu tika.
· Ko TEMED (N, N, N ', N '- tetramethyl ethylenediamine) he whakakorikori, i roto i te pounamu parauri, ka whakatakotoria ki roto i te pouaka whakamatao.Tāpirihia i mua tonu i te ringihanga kāpia.
· polyacrylamide electrophoresis whakamahia karaihe i roto i te electrophoresis me horoi i mua me muri i ia wa.I muri i te electrophoresis, horoia ki te paraihe ngohengohe me te kakahu i roto i te wai hopi wera, horoia ki te wai makuku ka tu kia maroke.
· te makuku me te puehu ka puta mai i te polymer hollow.I mua i te electrophoresis, horoia te pereti karaihe ki te kaihoroi karaihe ka horoia ki te paraihe ngohengohe.Horoi ki te wai makuku ka whakamaroke ki te muru pepa.Ko te horoi ki te 70% waiwaro i mua i te muru ki te pepa ka awhina ki te horoi me te tere ake te whakamaroke.Tāpirihia nga tauira o te acrylamide kia rite: BIS, wai, wairewa parepare, ammonium persulfate, TEMED.Ruperupea pai ka riringi tonu.
Kaore e tika kia whakakorehia te polyacrylamide i mua i te polymerization.(I whakamahia te Acrylamide ki roto i te korehau ki te tango mirumiru na te mea ka aukati te hāora i te polymerization.)
Ko nga tohutohu tauira tohu kapia whakapae.
· hoatu he pepa pango ki roto i te pouaka o raro, he pango te papamuri kia mahia he kohao tauira kia marama ake ai te kite.
· ki tonu i te taika kapia, ki runga noa i te colloid.
· Mēnā he rama te tapa, whakakāhia ngā rama, kia whiti te marama colloid.Tuhia te tauira ki roto i te pipette.
· te whakamahi i te taputapu paipa aunoa.
· i roto i te 10-200 mu 1 te waahi neke wai ka taea te whakamahi i te nuinga o nga tohu o te tauira.Mo nga kohao tauira iti rawa (iti iho i te 10μ1), he pai ake te mahunga pipette roa e whakamahia ana mo te kapia raupapa.
· ka rukuhia te paipa ki roto i te tauira, ka ngohia te wai rere.Ka ahua piri te tauira ki te glycerin, a ka kumea pea te mirumiru hau ki roto i te upoko pipette.
· Ko nga tauira i muri i te hoha i te upoko paipa, ka neke ata te upoko wē ki te taha o te paipa, ki te muru pepa ranei ka ngotea te upoko wē ki waho o ngā rūrūtia.Kia tupato kei ngotea te tauira.
Whakanohoia te tauira ki roto i te poka tauira
· he paipa paipa hei pupuri i te pehanga iti, kia paku te nuku o nga tauira ki te mahunga wai.
· ko te upoko paipa kua kuhua ki roto i te parapara, he paku teitei ake i nga rua wahi, kia mau tonu te pehanga pai.Ko te pito o te pipette ka taea te whakauru ki roto i tetahi poka iti.
· kia ata haere nga tauira ki waho.Ka whakanohohia te pito paipa ki runga ake i te kohao tauira, ka totohu te tauira ki roto i te rua.Tukua kia totohu te tauira ki te whakaki i te rua tauira kaua e pana ki roto.
· ka mutu te maturuturunga iho o te tauira whai mahunga wai, ka neke te wai ki te waewae tuarua, ka piki haere te nekehanga wai, ka neke atu i te parepare
Me pehea te mahi tauira kapia poutū?
· he kohao tauira tohu kapia poutū i hangaia i waenganui i nga wahanga karaihe e rua.I roto i te kapia tino kikokore, kaore e taea te kuhu te upoko pipette ki waenganui i nga papa karaihe e rua.Tirohia te glycerin!Whakanohoia te upoko pipette ki runga i te rua tauira ka totohu te tauira ki roto i te poka.
· tohu tauira i mua, kia mau ki te whakatakoto i te pito poutū o te poka tauira polypropylene acyl gel kua horoia kia ma.Horoia te acrylamide korepolymerized me te wai ka puta i raro o te rua tauira.Ma te wai ka tino iti ake te poka tauira.Whakamahia he syringe 25ml, 50ml ranei me te ngira 18 ine.Ringihia ki roto i te parapara hiko ka ata horoi i te poka tauira.
· he uaua te kite i te kohao tauira, engari i te wa ano, he ngawari te toenga.Mena he maha nga rua, ka taea te whakamatautau i te tauira parepare ki te puru bromophenol.
Te wa tuku: Maehe-31-2023