· polyacrylamideKo te reera me te monomer acrylamide, nga mea timatanga polymerization, te whakakorikori, me te tika o te tote me te ranunga parepare.
· acrylamideme te BIS (N, N '- methylene acrylamide rua) ko te ahua o te monomer matrix gel.
· te haukinia persulfate ka timata te tukanga polymerization whakapiri. Ko te hanga o te kapia me 10% te otinga haukinia persulfate kua rite ki te wai. Ko te nuinga o nga korero e tohu ana me kaha te whakamahi. Heoi, ko te otinga 10% ka taea te whakanoho ki te 4 ℃ mo etahi wiki kaore he mate nui. Hangaia kia 10 ml ka rukea ina kore te kapia e whakahiato.
Aki: Te ōrau oacrylamidei roto i te whakaraupapa kāpia me te kāpia pūmua e kore e rite. Mena kei te whakamahi i te acrylamide kua oti te hanga: te otinga BIS, me mohio ki te tiki i te pounamu tika.
· Ko TEMED (N, N, N ', N '- tetramethyl ethylenediamine) he whakakorikori, i roto i te pounamu parauri, ka whakatakotoria ki roto i te pouaka whakamatao. Tāpirihia i mua tonu i te ringihanga kāpia.
· polyacrylamide electrophoresis whakamahia karaihe i roto i te electrophoresis me horoi i mua me muri i ia wa. I muri i te electrophoresis, horoia ki te paraihe ngohengohe me te kakahu i roto i te wai hopi wera, horoia ki te wai makuku ka tu kia maroke.
· te makuku me te puehu ka puta mai i te polymer hollow. I mua i te electrophoresis, horoia te pereti karaihe ki te kaihoroi karaihe ka muru ki te paraihe ngohengohe. Horoi ki te wai makuku ka whakamaroke ki te muru pepa. Ko te horoi ki te 70% waiwaro i mua i te muru ki te pepa ka awhina ki te horoi me te tere ake te whakamaroke. Tāpirihia nga tauira o te acrylamide kia rite: BIS, wai, wairewa parepare, ammonium persulfate, TEMED. Ruperupea pai ka riringi tonu.
Kaore e tika kia whakakorehia te polyacrylamide i mua i te polymerization. (I whakamahia te Acrylamide ki roto i te korehau ki te tango mirumiru na te mea ka aukati te hāora i te polymerization.)
Ko nga tohutohu tauira tohu kapia whakapae.
· hoatu he pepa pango ki roto i te pouaka o raro, he pango te papamuri kia mahia he kohao tauira kia marama ake ai te kite.
· ki tonu i te taika kapia, ki runga noa i te colloid.
· Mēnā he rama te tapa, whakakāhia ngā rama, kia whiti te marama colloid. Tuhia te tauira ki roto i te pipette.
· te whakamahi i te taputapu paipa aunoa.
· i roto i te 10-200 mu 1 te waahi neke wai ka taea te whakamahi i te nuinga o nga tohu o te tauira. Mo nga kohao tauira iti rawa (iti iho i te 10μ1), he pai ake te mahunga pipette roa e whakamahia ana mo te kapia raupapa.
· ka rumakina noa te paipa ki roto i te tauira, ka ngohia te wai rere. Ka ahua piri te tauira ki te glycerin, a ka kumea pea te mirumiru hau ki roto i te upoko pipette.
· Ko nga tauira i muri i te hongi i te upoko paipa, ka neke ata te upoko wē ki te taha o te paipa, ki te muru pepa ranei ka ngotea te upoko wē ki waho o ngā rūrūtia. Kia tupato kei ngotea te tauira.
Whakanohoia te tauira ki roto i te poka tauira
· he paipa paipa hei pupuri i te pehanga iti, kia paku te nuku o nga tauira ki te mahunga wai.
· ko te upoko paipa kua kuhua ki roto i te parapara, he paku teitei ake i nga rua wahi, kia mau tonu te pehanga pai. Ko te pito o te pipette ka taea te whakauru ki roto i tetahi poka iti.
· kia ata haere nga tauira ki waho. Ka whakanohohia te pito paipa ki runga ake i te kohao tauira, ka totohu te tauira ki roto i te rua. Tukua kia totohu te tauira ki te whakaki i te rua tauira kaua e pana ki roto.
· ka mutu te maturuturunga iho o te tauira whai mahunga wai, ka neke te wai ki te waewae tuarua, ka piki haere te nekehanga wai, ka neke atu i te parepare
Me pehea te mahi tauira kapia poutū?
· he kohao tauira tohu kapia poutū i hangaia i waenganui i nga wahanga karaihe e rua. I roto i te kapia tino kikokore, kaore e taea te kuhu te upoko pipette ki waenganui i nga papa karaihe e rua. Tirohia te glycerin! Whakanohoia te upoko pipette ki runga i te rua tauira ka totohu te tauira ki roto i te poka.
· tohu tauira i mua, kia mau ki te whakatakoto i te pito poutū o te poka tauira polypropylene acyl gel kua horoia kia ma. Horoia te acrylamide korepolymerized me te wai ka puta i raro o te rua tauira. Ma te wai ka tino iti ake te poka tauira. Whakamahia he syringe 25ml, 50ml ranei me te ngira 18 ine. Ringihia ki roto i te putunga hiko ka ata horoi i te poka tauira.
· he uaua te kite i te kohao tauira, engari i te wa ano, he ngawari te toenga. Mena he maha nga rua, ka taea te whakamatautau i te tauira parepare ki te puru bromophenol.
Te wa tuku: Maehe-31-2023